kocuria palustris source

Background and objective: Bacteria of the Genus Kocuria are found in several environments and their isolation from foods has recently increased due to more precise identification protocols using molecular and instrumental techniques. Identification of the isolates was further confirmed by 16S rRNA gene sequence analysis. To examine the hypothesis that rice (Oryza sativa, cultivar Kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. of CDS No. Viability and purity assays of this product were performed at the time of production as part of quality control. Kocuria rhizophila DC2201 - BacMap JCM Catalogue The 2251-bp and 2488-bp circular genomes of pKPAL1 and pKPAL2, respectively, were sequenced. 14 Journal of Medical and Scientific Research CASe RePoRT Kocuria varians - An emerging cause of ocular infections Anita K videkar1,*, Pranathi B 1, Madhuri Gadde and Nashrah Nooreen1 1Department of Ophthalmology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad Abstract Purpose: We report a rare case of recurrent conjunctivitis caused by gram positive aerobic microorganism Binds to 23S rRNA. Kocuria - Wikipedia PDF Public Notice - United States Army nov., an actinobacterium isolated from the Indian Himalayas S. Mayilraj,1 R. M. Kroppenstedt,2 K. Suresh1 and H. S. Saini3 1 Correspondence Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, S. Mayilraj Sector 39-A . Comprehensive and Live Air Purification as a Key ... Kocuria palustris sp. as a single energy and carbon source, in a 200 ml conical flask containing 50 ml mineral salts (MS) medium with the . 2008 ), agricultural soil (bachate et al. Solid waste dates (SWD) and corn steep liquor (CSL) as agro-industrial products were performed to enhance the performance of Kocuria sp. Secondary sources include animal and dairy products. This strain was identified as K. palustris based upon analyses of cellular chemical compositions (cellular fatty acids, isoprenoides, quinones, and sugars), patterns of carbon source, average nucleotide identity of genome . nov., iso-lated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). nov., a novel actinobacterium isolated from saline, alkaline desert soil in Egypt. Case presentation An 18 year old male was brought to a tertiary care hospital in Greater Noida in an Kocuria kristinae is a facultative anaerobic, non-motile, gram positive coccus occurring in irregular clusters and tetrads. Currently, there are only limited reported cases of . The structure herein . During bacterial growth, high emulsifying International Journal of Systematic and Evolutionary Microbiology (2006), 56, 1971-1975 DOI 10.1099/ijs..63915- Kocuria himachalensis sp. rosea) using the Vitek 2 system has been reported in the literature. The . West Coast Chapter PDA . Two cryptic plasmids, designated pKPAL1 and pKPAL2, were identified from the gram-positive bacterium Kocuria palustris IPUFS-1, which was isolated from a fish source. Cells were Gram-positive coccoid and formed no endospores. Due to addition of 0.5 mg ml-1 yeast extract as additional carbon source, residual naphthalene extracted was 41 and 55% . It has been found in the milk of water deer and reindeer. Performance of Isolated Kocuria sp. 1995 Conduct genome-based taxonomy of genus at TYGS nov., isolated from the uropygial gland of an American barn owl ( Tyto furcata ). Mariano et al . Kocuria rhizophila (strain ATCC 9341 / NBRC 12708 / DC2201) is a coccoid, halotolerant (tolerated up to 10% NaCl in growth media), phenol-degrading Gram-positive bacterium isolated from the rhizosphere of narrowleaf cattail (Typha angustifolia). 2014 ), freshwater (adrian et al. Medical Information Search Braun MS, Wang E, Zimmermann S, Wagner H, Wink M. Kocuria tytonis sp. Kocuria rhizophila DC2201. To download a certificate of analysis for Kocuria rhizophila Kovacs et al. Kocuria palustris sp. As well as Kocuria species being misidentified by phenotypic tests, misidentification of CoNS (S. epidermidis/ Staphylococcus haemolyticus, of which 58 % are phenotypi-cally variable) as Kocuria species (K. varians/K. The present invention provides a reliable and specific means for the detection of microorganisms of the taxonomic unit set forth by the genera Micrococcus, Kocuria, Kytococcus, Nesterenkonia , which optionally also allows for further determination and discrimination of other microorganisms of other taxonomic units and of one or more genera or species within the genera Micrococcus, Kocuria . Kocuria palustris TAGA27 is an aerobe, mesophilic bacterium that was isolated from rhizosphere of Typha angustiflora. nov., isolated from the rhizoplane of the . August 2014 www.friedmanconsultingllc.com 410 . source are credited. Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. Members of the species Kocuria rhizophila, belonging to the family Micrococcaceae in the phylum Actinobacteria, have been isolated from a wide variety of natural sources, such as soil, freshwater, fish gut, and clinical specimens. Most Corynebacterium are not lipophilic, and can be either fermentative or non-fermentative. 1995 Assigned by: Tvrzova L, Schumann P, Sedlacek I, Pacova Z, Sproer C, Verbarg S, Kroppenstedt RM. Kocuria kristinae. are gram-positive, aerobic microorganisms and found as normal skin flora in humans and other mammals. Kocuria is a genus of gram-positive bacteria in the phylum Actinobacteria, including the sequenced species Kocuria rhizophila. This review describes biotechnological properties and food-linked aspects of these bacteria, which are closely associated with clinical cases. We aimed at determining whether the gut microbiota of sunbirds harbors bacterial species that enable the birds to cope with these toxic alkaloids. The producer actinobacteria strains correspond to two Kocuria spp. 4. (2008) isolated Kocuria palustris from soil of a petrol pump and first time reported degradation of commercial diesel oil by K. palustris . 1995 Category: Genus Proposed as: gen. nov. Etymology: Ko.cur'i.a. when provided with 0.5 mg ml-1 concentration as sole carbon source. are aerobic, grow best at 30°C - 35°C, and are slow growing. Emerging Bacterial Infection: Identification and Clinical Significance of Kocuria Species Venkataramana Kandi , Padmavali Palange , Ritu Vaish , Adnan Bashir Bhatti , Vinod Kale , Maheshwar Reddy Kandi , Mohan Rao Bhoomagiri 1. and one Micrococcus sp., that have been identified after phylogenetic analysis as the strains Kocuria marina F-276,310, Kocuria palustris F-276,345, and Micrococcus yunnanensis F-256,446 (Figure 1). On the basis of differential phenotypic characteristics and genotypic distinctiveness, strain S9-65T should be classified as representative of a . Three of these bacteria — Kocuria palustris, Bacillus pumilus and Bacillus subtilis — utilized polyethylene as their sole source of carbon and energy. This strain was identified as K. palustris based upon analyses of cellular chemical compositions (cellular fatty acids, isoprenoides, quinones, and sugars), patterns of carbon source, average nucleotide identity of genome and digital DNA-DNA relatedness. The positive isolates were identified as Kocuria palustris M16, Bacillus pumilus M27 and Bacillus subtilis H1584 based on the 16S rRNA gene sequence homology. Ars operon has been widely studied . Further elaborate study, especially the multicentric studies may help us in designing future course of action. and one Micrococcus sp., that have been identified after phylogenetic analysis as the strains Kocuria marina F-276,310, Kocuria palustris F-276,345, and Micrococcus yunnanensis F-256,446 . K. rhizophila is important from an industrial viewpoint, because the bacterium grows rapidly with high cell density and exhibits robustness at various growth conditions. Arbitrarily primed PCR analysis showed a clonal strain. The Kocuria cultures were also used in lab either before or after we did a streak of our isolates. We describe five patients with positive blood culture for Kocuria species. In the United States, the Center for Disease Control (CDC) estimates that more than 1.7 million people suffer from an infectious complication annually, representing between 5 and 10% of all hospital admissions and costs ranging . The single, circular chromosome of . fem. nov. and Kocuria rhizophila sp. Conditions for Growth: Corynebacterium spp. This species can cause diseases not only in immunosuppressed patients [5], but also skin infection [16] and lung infection [6] in immunocompetent patients. nov. Int J Syst Evol Microbiol 2005; 55:139-142. A bacterial strain named DDK6 was isolated from diesel-contaminated soil from a petrol station in Al-Hofuf city, after enrichment on diesel oil. More recent studies have shown that several species of Staphylococcus, Micrococcus, Kocuria, Arthrobacter, Brachybacterium, Brevibacterium, Corynebacterium, Curtobacterium, Microbacterium and Rhodococcus are also present on the . 2011 ), marine sediments (dastager et al. carniphila, Kocuria flava, Kocuria palustris, Kocuria halotolerans, Kocuria himachalensis, Kocuria koreensis, Kocuria kristinae, . Species description is based on a single isolate. aerobe mesophilic 16S sequence Bacteria genome sequence Name and taxonomic classification Morphology Culture and growth conditions Physiology and metabolism [Ref. The results indicate that a diverse community of pigmented Gram-positive bacteria is associated with Antartic macroalgae and suggest its potential as a promising source of antimicrobial and pigmented . Kocuria kristinae. Unreviewed-Annotation score: -Protein inferred from homology i. As carbon sources, we tested the diesel oil (W-D) recovered from the groundwater at a petrol station [whose soil was the source of the cultures Staphylococcus hominis and Kocuria palustris (Mariano et al., 2007)] and, comparatively, a commercial diesel oil (C-D) purchased from a local petrol station. A new thiazolyl peptide, kocurin (1), was isolated from culture broths of a marine-derived Kocuria palustris. Kocuria rhizophila and Kocuria palustris. 2016 ), sewages (abbas et al. Thursday, August 21, 2014 . K. palustris [15], K. marina [16], K.rosea [17], indicating . El-Gendy 3 and Hussein Nassar 3 of tRNA DC2201 Soil 2.70 1 71.2 2,357 3 46 P7-4 Intestine of rabbitfish (Siganus doliatus) 2.82 75 70.4 2,370 5 46 Generally considered non-pathogenic, commensal bacteria, these organisms are normal flora of the skin, oral cavity, and mucous membranes of humans and animals. It is possible that Kocuria rhizophila came from the gym, or that it was a contaminant in my plate that was mistaken for a colony from the original swab. Kocuria is a member of the Micrococcus family, which includes Kocuria rosea, Kocuria kristinae, Kocuria varians, Kocuria palustris, Kocuria rhizophila, Kocuria marina and Kocuria . South San Francisco, CA . Kocuria. Isolation source Samples No. of Contig (s) G+C (%) No. They usually form 2-3 mm whitish, small, round, raised, convex colonies on initial isolation and might develop non-diffusible yellowish pigmentation after prolonged incubation, as shown in Figure Figure11. K. palustris K. rhizophila Strains No. spike-rush (Eleocharis palustris), sump week (Iva annua), and fragrant flat sedge (Cyperus odoratus ). Forms part of two intersubunit bridges in the 70S ribosome. 2014 ), clinical samples (tewari et al. Strain Source Size (Mb) No. This bacterium was first identified and described by Miroslav Kosur, a Slovakian microbiologist. Members of the family are defined by a wide range of morphological and chemotaxonomic properties, such as polar lipids, fatty acids, amino acids of peptidoglycan, and whole-cell sugars which are used for the delineation of genera and species. Kocuria spp. Isolation % Strains No. The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data. Endophytic bacteria are considered to originate from the external environment. Healthcare organizations strive to provide optimal patient experience by improving care quality and enhancing clinical outcomes, while containing associated costs. peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis (CAPD). A case of persistent bloodstream infection with Kocuria rhizophila related to a damaged central venous catheter in a 3-year-old girl with Hirschsprung's disease is reported. Presented here is the first completely assembled genome sequence of Kocuria palustris , an actinobacterial species with broad ecological distribution. Kocuria aegyptia sp. Function i Caution. respectively, in LB/mineral media. The family Micrococcaceae is well-defined family within the order Actinomycetales. Kocuria is a genus of gram-positive bacteria. K. palustris DSM 15639, YIM 70083 K. palustris T2_20, DSM 30987 K. palustris CCUG 49552 Isolation source Rhizoplane of the narrow-leaved cattail, Typha angustifolia, inhabiting a floating mat on the Soroksar tributary of the Danube river . Presented here is the first completely assembled genome sequence of Kocuria palustris, an actinobacterial species with broad ecological distribution. Frequent questions. Cultural characteristics of Kocuria Kocuriaspp do not produce hemolysis on blood agar, unlike most clinical isolates of Staphylococci. Kocuria palustris. Environmental Monitoring Fundamentals . Kocuria is a heterogeneous bacterial genus and widely distributed in diverse habitats [1]. sequences of SAR1 with sequences obtained by doing a Blast searching revealed 96% similarity to Kocuria palustris strain TAGA27. Name: Kocuria Stackebrandt et al. Int J Members of . Micrococcus/Kocuria are the most commonly occurring aerobic bacteria in cleanroom environments. ALTERNATIVES: During project planning, the applicant considered three alternatives in an During bacterial growth, high emulsifying The single, circular chromosome of K. palustris MU14/1 comprises 2,854,447 bp, has a G+C content of 70.5%, and contains a deduced gene set of 2,521 coding sequences. Gram-positive cocci, 1.0-1.5 μm in diameter, occuring in pairs, tetrads and clusters. species are bacteria often found in the en-vironment and human skin. are common in nature as well as the normal skin flora and mucosa of humans and animals. The strain was identified as K. rhizophila by 16S rRNA gene sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry. Department of Microbiology, Prathima Institute of Medical Sciences 2. Kocuria palustris was reported as another As-tolerant bacterium containing As-detoxifying genes indicating that such mechanisms may be common in genus Kocuria. Strain: Kocuria palustris PEL Bio Project: PRJNA340288 Environmental: tropical … Isolated aerobic bacteria were able to bioaccumulate 137 Cs and 60 Co in laboratory experiments. nasal colonization of streptococcus. The only known source of Brev. In Brazil, M. fortuitum was found in raw milk samples from the States of São Paulo [14] and Paraná [15]. Currently, there are more than 18 species of Kocuria identified based on the 16S rRNA phylogenetic studies. Isolation % Thalassemia patients 70 1 1.4 9 12.8 Healthy persons 20 2 10.0 5 25.0 Sum 90 3 14 Figure 1: Phenetic dendrogram for strains related to K. palustris and K. rhizophila built by single linkage method and nearest neighbor joining with simple . An endophytic Kocuria palustris strain harboring multiple arsenate reductase genes . SAR1 in crude oil biodegradation. SAR1 in crude oil biodegradation. SAR1 in Light Crude Oil Biodegradation Abdullah M. El Mahdi 1 , Hamidi Abdul Aziz 1 , 2 * , Salem S Abu Am 1 , Nour Sh. Parent taxon: Kocuria Stackebrandt et al. Orange-tufted sunbirds (Cinnyris osea) feed on the nectar of the tobacco tree (Nicotiana glauca) which contains toxic pyridine alkaloids characterized by high concentrations of anabasine and much lower concentrations of nicotine. et al. The active isolates were phylogenetically related to Agrococcus baldri, Brachybacterium rhamnosum, Citricoccus zhacaiensis and Kocuria palustris. Int J Syst Bacteriol 1999; 49: 167-173. Editor: Kocuria. Kocuria has been identified in the milk of water deer and reindeer. CASE REPORT The expanding spectrum of human infections caused by Kocuria species: a case report and literature review Shashikala Purty1,*, Rajagopalan Saranathan2,*, K Prashanth2, K Narayanan3, Johny Asir1, Chandrakesan Sheela Devi1 and Satish Kumar Amarnath4 Although not previously known to cause human infections, Kocuria species have now emerged as human pathogens, mostly in palustris and K. rhizophila [2]. Members of the genus Kocuria were isolated from a wide variety of natural . Its structural elucidation was accomplished using a combination of spectroscopic and chemical methods, including HRMS, extensive 1D and 2D NMR analysis, MS/MS fragmentation, and chemical degradation and Marfey's analysis of the resulting amino acid residues. To identify new microbes that can degrade plastic, the researchers cultivated 60 species of marine bacteria on low-density polyethylene films isolated from pieces of polyethylene bags. linens is the surface of smear-ripened cheeses and, at one time, it was considered to be the dominant bacterium found on these cheeses. [3] Kocuria. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. kocuria, a genus of gram-positive bacteria, has been isolated from a variety of resources, including air (zhou et al. Kocuria palustris IPUFS-1 (Toda et al., 2017) was used as a source of cryptic plasmids. The weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the M16, M27 and H1584 isolates, respectively. 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